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Far-Field Optical Nanoscopy

Gebonden Engels 2015 2015e druk 9783662455463
Verwachte levertijd ongeveer 9 werkdagen

Samenvatting

This book describes developments in the field of super-resolution fluorescence microscopy or nanoscopy. In 11 chapters, distinguished scientists and leaders in their respective fields describe different nanoscopy approaches, various labeling technologies, and concrete applications. The topics covered include the principles and applications of the most popular nanoscopy techniques STED and (f)PALM/STORM, along with advances brought about by fluorescent proteins and organic dyes optimized for fluorescence nanoscopy. Furthermore, the photophysics of fluorescent labels is addressed, specifically for improving their photoswitching capabilities. Important applications are also discussed, such as the tracking and counting of molecules to determine acting forces in cells, and quantitative cellular imaging, respectively, as well as the mapping of chemical reaction centers at the nano-scale.

The 2014 Chemistry Nobel Prize® was awarded for the ground-breaking developments of super-resolved fluorescence microscopy. In this book, which was co-edited by one of the prize winners, readers will find the most recent developments in this field.

Specificaties

ISBN13:9783662455463
Taal:Engels
Bindwijze:gebonden
Aantal pagina's:329
Uitgever:Springer Berlin Heidelberg
Druk:2015

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Inhoudsopgave

<p>Part I: Optical Nanoscopy Techniques</p><p> STED Fluorescence Nanoscopy<br>Christian Eggeling, Stefan W. Hell</p><p> Super-Resolution Imaging Through Stochastic Switching and Localization of Single Molecules: An Overview<br>Ke Xu, Sang-Hee Shim, and Xiaowei Zhuang</p><p> A Practical Guide to dSTORM: Super-Resolution Imaging with Standard Fluorescent Probes<br>Markus Sauer</p><p> Part II: Labelling Technology for Optical Nanoscopy</p><p> Single-Molecule Photocontrol and Nanoscopy<br>Matthew D. Lew, Steven F. Lee, Michael A. Thompson, Hsiao-lu D. Lee, and W. E. Moerner</p><p> Probes for Nanoscopy: Fluorescent Proteins<br>Susan Gayda, Per Niklas Hedde, Karin Nienhaus, and G. Ulrich Nienhaus</p><p> Tailoring Fluorescent Labels for Far-Field Nanoscopy<br>Dmytro A. Yushchenko and Marcel P. Bruchez</p><p> Probes for Nanoscopy: Photoswitchable Fluorophores<br>Pedro F. Aramendia and Mariano L. Bossi<p> Far-Field Nanoscopy with Conventional Fluorophores: Photostability, Photophysics, and Transient Binding<br>Thorben Cordes, Jan Vogelsang, Christian Steinhauer, Ingo H. Stein, Carsten Forthmann, Andreas Gietl, Jürgen J. Schmied, Guillermo P. Acuna, Sebastian Laurien, Birka Lalkens, and Philip Tinnefeld</p><p> Part III: Developments and Applications of Optical Nanoscopy</p><p> NASCA Microscopy: Super-Resolution Mapping of Chemical Reaction Centers<br>Gert De Cremer, Bert F. Sels, Dirk E. De Vos, Johan Hofkens, and Maarten B.J. Roeffaers</p><p> Counting Molecules: Toward Quantitative Imaging<br>Maximilian H. Ulbrich</p><p> In Vivo Tracking of Single Biomolecules: What Trajectories Tell Us About the Acting Forces<br>Mario Brameshuber and Gerhard J. Schütz</p><p> </p><p> </p>

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